![]() ![]() The Col-0 plant was transformed by the floral dip method. The METHOD OF ENGINEERING CHEMOTHERAPY DRUG RESISTANT T-CELLS FOR IMMUNOTHERAPY patent was assigned a Application Number 15037988 by the United States Patent and Trademark Office (USPTO). In this study, ADAP was proposed as a negative regulator in aliphatic GSL biosynthesis due to the over-expression of downstream aliphatic GSL genes (UGT74C1 and IPMI1) in ADAP-SRDX line. Till now, SRDX repressor domain was the most frequently used TRD in plant. Molecular phenotypes include altered epidermal cell patterning and altered cell-cycle gene expression compared to wildtype.ĪtTCP23 coding sequence without stop codon was fused to the SRDX repressor domain (EAR peptide sequence LDLDLELRLGFA) with terminator codon TAA, and cloned into pK2GW7. Expression of AtTCP15SRDX, AtTCP15 fused with a SRDX repressor domain, induces extra endoredupli- cation in trichomes and cotyledon cells in transgenic. By applying CRES-T, ADAP TF gene was fused to a plant-specific EAR-motif repressor domain (SRDX), which suppresses the expression of ADAP target genes. Consistent with this interpretation, fusion of the repressor domain SRDX to WUSm1 (SRDX-WUSm1) restored complementation of lam1 (Fig. Furthermore, the fusion of synthetic transcriptional repressor domain (TRD) to dCas9 could improve the gene silencing efficiency dramatically, the above all was also known as CRISPR interference system (CRISPRi). ![]() Upward growth of the leaves and smaller rosette size plants have shorter pedicels with irregularly shaped larger sepals, shorter petals, and less trichomes compared to wildtype. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |